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At this point, we will look into the evolution of blade systems at a deeper level, including a detailed history and the phases of evolution.

The decrease of oxygen reduction current measured with the Clark oxygen sensor indicates the concentration of glucose. Enzyme electrodes belong to the family of biosensors. These also include systems with tissue sections or immobilized microorganism suspensions playing an analogous role as immobilized enzyme layers in enzyme electrodes. While the stability of enzyme electrode systems is the most difficult problem connected with their practical application, this is still more true with the bacteria and tissue electrodes.

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Generally, a diblock (AB) or triblock (ABA) copolymer in solution can selfassemble into micelles if the solvent is good for one block but bad for the other, ie, being block selective The micellar aggregates can have various morphologies (star, crew cut, rod, and vesicles), depending on a number of parameters such as the relative lengths of the blocks, polymer concentration, and the used solvent (Discher and Eisenberg, 2002) If the BCP is amphiphilic, ie, being composed of a hydrophilic and a hydrophobic (lipophilic) polymer, its micelles in aqueous solution have a compact core region formed by the hydrophobic block and a shell (corona) formed by the hydrophilic block, which ensures their solubility (or dispersion) in water.

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Ammann, D., Ion-Selective Microelectrodes, Springer-Verlag, Berlin, 1986. Baucke, F. G. K., The glass electrode Applied electrochemistry of glass surfaces, J. Non-Crystall. Solids, 73, 215 (1985). Berman, H. J., and N. C. Hebert (Eds), Ion-Selective Microelectrodes, Plenum Press, New York, 1974. Cammann, K., Working with Ion-Selective Electrodes, Springer-Verlag, Berlin, 1979. Durst, R. A. (Ed.), Ion-Selective Electrodes, National Bureau of Standards, Washington, 1969. Eisenman, G. (Ed.), Glass Electrodes for Hydrogen and Other Cations, M. Dekker, New York, 1967. Freiser, H. (Ed.), Analytical Application of Ion-Selective Electrodes, Plenum Press, New York, Vol. 1, 1978; Vol. 2, 1980. Janata, J., The Principles of Chemical Sensors. Plenum Press, New York, 1989. Koryta, J. (Ed.), Medical and Biological Applications of Electrochemical Devices, John Wiley & Sons, Chichester, 1980. Koryta, J., and K. Stulik, Ion-Selective Electrodes, 2nd ed., Cambridge University Press, Cambridge, 1983. Morf, W. E., The Principles of Ion-Selective Electrodes and of Membrane Transport, Akademiai Kiado, Budapest and Elsevier, Amsterdam, 1981. Scheller, F., and F. Schubert, Biosensors, Elsevier, Amsterdam, 1992. Sykova, E., P. Hnik, and L. Vyklicky (Eds), Ion-Selective Microelectrodes and Their Use in Excitable Tissues, Plenum Press, New York, 1981. Thomas, R. C , Ion-Sensitive Intracellular Microelectrodes. How To Make and Use Them, Academic Press, London, 1978. Turner, A. P. F., I. Karube and G. S. Wilson, Biosensors, Fundamentals and Applications, Oxford University Press, Oxford, 1989. Zeuthen, I. (Ed.), The Application of Ion-Selective Microelectrodes, Elsevier, Amsterdam, 1981.

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Before They Were Called Blades (pre-1999)

Here we emphasize that while core shell micelle formed by a diblock copolymer will be used in the discussion hereafter, the principles are applicable to other morphologies of micellar aggregates and to triblock copolymers as well As with micelles of small-molecule surfactants, the decisive parameter that controls the noncovalent micellar association of BCPs is the hydrophilic hydrophobic balance, ie, the relative importance of the two blocks, which is mainly determined by their relative lengths (BCP composition) and their different solubilities in water (intermolecular interactions with water determined by the chemical structures of the two blocks) In the context of polymer nanocarriers for controlled delivery applications as explained earlier, lightcontrollable BCP micelles are those that can exhibit a structural or morphological change, such as dissociation, disintegration, and swelling, upon interaction with light to allow the release of loaded guest molecules into the aqueous solution.

All living organisms consist of cells, and the higher organisms have a multicellular structure. In addition, the individual cells contain specialized formations called organelles like the nucleus, the mitochondria, the endoplasmatic reticulum, etc. (see Fig. 6.7). A great many life functions occur at the surfaces of cells and organelles, such as conversion of energy, the perception of external stimuli and the transfer of information, the basic elements of locomotion and metabolism. The large internal' surface of the organism at which these processes occur leads to their immense variety. The surfaces of cells and cellular organelles consist of biological membranes. In contrast to those discussed in the previous text these membranes are incomparably thinner. Electron microscope studies show them to consist of three layers with a thickness of 7-15 nm (see Fig. 6.8). In 1925, E. Gorter and F. Grendel isolated phospholipids from red blood cells and spread them out as a monolayer on a water-air interface. The overall surface of this monolayer was approximately twice as large as the surface of the red blood cells. The authors concluded that the erythrocyte membrane consists of a

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